The Mechanism Research of Curcumin in Alleviating the Renal Injury Induced by Calcium Oxalate Crystals
Abstract：Objective: To observe the effect of curcumin in kidney injury induced by calcium oxalate crystals,and to investigate the underlying mechanism of renoprotective effect of curcumin. Methods: C57BL /6J mice intraperitoneally injected with glyoxylate and HK-2 cells exposed to calcium oxalate monohydrate were taken as calcium oxalate-induced kidney damage models. Curcumin was added as the interventional factor respectively. V0N KOSSA staining was used to observe renal oxalate calcium deposition, and immunochemistry、 qRT-PCR and Western blot were used to monitor the changes of epithelial-mesenchymal transition(EMT)markers(E-cadherin、 N-cadherin and Vimentin). Results: (1)VON KOSSA staining showed that curcumin could significantly reduce the black calcium deposition in mouse kidney tissue. (2)Immunochemistry、 qRT-PCR and Western blot witnessed that calcium oxalate decreased the expression of E-cadherin and increased the expression of N-cadherin as well as Vimentin in vivo and in vitro. However, curcumin remarkably alleviated these changes showing above of EMT markers. Conclusion: Curcumin can reduce the glyoxylate induced kidney calcium crystal formation and deposition,and the possible mechanism may be related the regulatory effects on EMT.
Keywords：Calcium oxalate crystalCurcuminEMTRenal calculus
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 852-855,941 )