Effects of Zhuangtong Drinking on Endogenous Neural Stem Cells in Rats with Cerebral Infarction
Chen Xiaofeng
Wei Liuyan
He Weina
Liu Yanping
Li Kaifeng
He Qianchao
Liu Qiang
Abstract：Objective To observe the effect of Zhuangtong drinking(ZTD)on the proliferation and differentiation of endogenous neural stem cells (NSC)in cerebral ischemia.Methods Two hundreds and forty clean and healthy male Sprague-Dawly (SD)rats were randomly divided into model group,sham operation group,ZTD treatment group and normal group.The middle cerebral artery occlu-sion(MCAO)model was established by thread inserting method.The rats were killed in postoperative 1 d,3 d,7 d,14 d,21 d,28 d. Immunohistochemistry was used to detect the positive expression of neuronal core antigen(NeuN)and collagen fibrillary acidic pro-tein (GFAP)in the infarcted area and the corresponding area in each group.Results A large number of positive cells marked by Ne-uN and GFAP in the infarction area and corresponding to the area were observed in model group.Compared with sham operation group,the number of GFAP positive cells increased while the number of NeuN positive cells decreased in model group and ZTD treat-ment group(P<0.05).Compared with model group,the number of GFAP positive cells and NeuN positive cells increased (P<0.05), which increased to the top in the 14th day.Conclusion ZTD can promote the proliferation of endogenous NSC after cerebral infarc-tion,and induced to differentiate into neurons,astrocytes,oligodendrocytes,and so on.But the specificity is not obvious.New differen-tiated neurons keep the normal excitability and connections with other neurons,which suggestes that the neurons induced by ZTD can play a normal nerve function.
Keywords：cerebral infarctionZhuangtong drinkingendogenous neural stem cellsneuronal core antigencollagen fibrillary acidic protein
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 408-411 )