Panax Notoginseng Saponins Prevent Against Isoproterenol-induced Atrial Fibrillation In C57BL/6 Mouse:a Mechanism Study

Jia Chenglin; Chen Yu; Zhang Teng

doi:10.3969/j.issn.1672-1349.2018.03.010

Abstract：Objective To provide experimental evidence supporting the clinical application of Panax Notoginseng Saponins (PNS) as an anti-arrhythmic agent.To evaluate the effect and the possible mechanism of PNS on isoproterenol (ISO)-induced atrial fibrillation in mouse.Methods Fifty-four C57BL/6 male mice were randomly divided into experimental groups including vehicle-treated normal controls,ISO-challenged controls,and PNS-treated ISO-challenged mice,respectively.ISO was administered to vehicle-treated or PNS-treated mice 30 min after PBS vehicle or PNS treatment.The dynamic changes of electrocardiogram were monitored in each group (n =6).And other mice were sacrificed 1.5 hours after ISO administration and the heart sections were measured by H&E staining and Annexin V fluorescent dyes to detect apoptosis.Further,the expression of miR-29b,miR-499,miR-210,and miR-328 in the mice heart were analyzed by real-time PCR.Results Compared to that from vehicle-treated ISO-challenged mice,the atrial fibrillation was significantly decreased in ISO-challenged mice treated by PNS.Moreover,compared to that from ISO-challenged mice treated by PBS vehicle,PNS treatment resulted in significant reductions in cell apoptosis.Furthermore,significantly upregulated expression of miR-499 and miR-210,and downregulated expression of miR-29b and miR-328 was observed in heart in vehicle-treated ISO-challenged controls compared to that from normal controls.Compared to vehicle-treated ISO-challenged controls,the expression of miR-499 and miR-210 were significantly downregulated,and the expression of miR-29b and miR-328 were significantly upregulated in PNS-treated ISO-challenged group.Conclusion Our work here demonstrated that PNS treatment prevent against isoproterenol-induced atrial fibrillation in C57BL/6 mouse,and its mechanism might be possibly associate with regulating expression of miRNAs,then improving the atrial electrical remodeling and structural remodeling.