The Effect of Leech Injection and Ischemic Postconditioning on the Expression of Phosphoinositide-3-kinase against Ischemia-reperfusion Injury in Rats
Hu Yueqiang
Lan Peng
Qin Hongling
Su Jinxun
Tan Qin
Abstract：Objective To observe the effect of leech injection and ischemic postconditioning (IPOC) on the expression of phosphoinositide-3-kinase (PI3K) mRNA and protein against ischemia-reperfusion (I/R) injury in rats.Methods Forty male Sprague-Dawly (SD) rats were randomly divided into four groups.:sham-operation (SO) group,middle cerebral artery occlusion (MCAO) group,IPOC group,and leech injection postconditioning (P-L) group.The IPOC and MCAO rat models were established by suture occlusion method.The volume of cerebral infarction was calculated by 2,3,5-Triphenyltetrazolium chloride (TrC) staining.The ex pression of PI3K mRNA and protein was detected by real-time fluorescence quantitative polymerase chain reaction (PCR) and western blot.Results The infarct volume of brain tissue was significantly lower in IPOC group than that in MCAO group after 24 h following I/R,which was lower in P-L group than that in IPOC group (P ＜0.05).The expression of PI3K mRNA and protein increased at 24 h following reperfusion in MCAO group (P ＜0.01).The expression of PI3K increased in IPOC group and P-L group (P ＜0.05 or P ＜0.01),which was higher in P-L group than that in IPOC group (P ＜0.05).Conclusion IPOC could protect neurons through upregulating the expression of PI3K.Leech injection postconditioning could augment the neuroprotection induced by IPOC.
Keywords：ischemia-reperfusion injuryischemic postconditioningleech injectionphosphoinositide-3-kinase
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 158-161 )