Effect of Curcumin on Myocardial Ischemia/reperfusion Injury and its Mechanism
Sun Meng
Xu Kun
Guo Min
Shi Hongtao
Han Qinghua
Jia Yongping
Wang Rui
Abstract：Objective To observe the protection of curcumin on myocardial ischemia/reperfusion injury,and to explore its possible mechanism.Methods H9C2 cardiomyocytes were cultured in vitro to simulate myocardial ischemia reperfusion(IR).lt was observed whether curcumin preconditioning could alleviate myocardial IR injury.The cardiomyocytes were divided into control group,IR group,curcumin (2.5μmol,5 μmol,10μmol) + IR group.The survival rate of cardiomyocytes was measured by MTT assay.The protective effects of SIRT3 on cardiomyocytes injured by IR after curcumin preconditioning were observed.The cardiomyocytes were divided into IR group,curcumin+ IR group,curcumin+ 3-TYP [sirtuin 3 (SIRT3) inhibitor] + IR group,3-TYP+ IR group.The apoptosis of cardiomyocytes was detected by TUNEL method.The expression of SIRT3,AcSOD,BCI-2 and BAX were measured by western blotting.Superoxide (SOD) activity,the contents of glutathione (GSH-Px),and malondiadehyde (MDA) were detected by oxidative stress kits.Results Compared with IR group,the cell survival rate significantly increased in curcumin (2.5 μmol,5tμmol,10 μmol) group (P ＜ 0.01).The cell viability increased in curcumin 5μmol group and 10 μmol group compared with curcumin 2.5μmol group (P ＜0.01).Compared with control group and IR group,myocardial apoptosis index decreased in curcumin group (P ＜0.01).Compared with the IR group,the activities of SOD and GSH-Px were significantly increased in the curcumin + IR group and curcumin + 3-TYP+ IR group (P＜0.01).The level of MDA was significantly decreased in curcumin + IR group (P ＜0.01).Compared with curcumin + IR group,the levels of MDA were significantly increased in curcumin + 3-TYP+ ischemia group and 3-TYP+ ischemia group (P ＜0.01).Compared with IR group,the expression of BCI-2 and SIRT3 in the curcumin+ IR group increased significantly (P ＜0.01),and the expression of Bax and AcSOD was significantly decreased (P ＜0.01).Compared with the 3-TYP pretreatment group,the expres sion level of SIRT3 and acetylated SOD was reversed (P ＜0.01).Conclusion Curcumin can protect myocardium against IR injury by up-regulating AcSOD level and promoting SIRT3 expression.
Keywords：ischemia-reperfusioncurcuminsirtuin 3myocardial cellsuperoxideglutathionemalondiadehyde
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 147-152 )