Protective Mechanism of Tongxinluo Capsule on Inflammatory Responses Induced by Degranulation of Mast Cells against Myocardial Ischemia Reperfusion Injury In Rats
Huang Ting
Li Shaodan
Li Han
Zhang Junxiu
Gao Lu
Liu Yi
Yang Minghui
Abstract：Objective To investigate the effect of protective effect and mechanism of Tongxinluo capsule on myocardial injury induced by mast cells in rats with ischemia reperfusion injury.Methods Thirty-six healthy adult male Sprague-Dawly (SD) rats were randomly divided into sham operation group,model group,Tongxinluo group.Myocardial ischemia reperfusion model was established by ligation of the left anterior descending coronary artery,rat myocardial ischemia 1 h,reperfusion 2 h;while the sham group was punctured without ligation.The rats in Tongxinluo group were intragastric administration by Tongxinluo suspension,and the model group and the sham group were intragastric administration by saline.After 1 weeks,the expressions of tryptase,c-Fos,and histamine were detected by immunohistochemistry.ELISA method was used to detect serum TPS and c-Fos,and TUNEL apoptosis index.Results The apoptosis index of the Tongxinluo group rats,the expressions of Tryptase,c-Fos,and histamine were significantly lower than those in the model group (P ＜ 0.01).Conclusion Tongxinluo capsule can inhibit the apoptosis of myocardial cells,and by reducing Tryptase,c-Fos and histamine,Inhibition of inflammatory response induced by degranulation of mast cells,reduce myocardial injury,thus protecting the myocardial tissue.
Keywords：myocardial ischemia reperfusionTongxinluo capsulemast cell degranulationinflammatory reactiontryptasec-Foshistamine
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2973-2976 )