Protective Effects of Shexiang Tongxin Dropping Pill on Microcirculation Dysfunction in Myocardial Infarction Mice Induced by LPS and Its Mechanism
Zhang Yanda
Shi Shanlan
Li Na
He Zhiqing
Wu Zonggui
Liang Chun
Abstract：Objective To explore the protective effects of Shexiang Tongxin dropping pill (STDP) on microcirculation dysfunction in mice and its mechanism.Methods Thirty male C57 mice were used,of which were randomly divided into sham control group,myocardial infarction (MI) + lipopolysaccharides (LPS) group,and STDP group.The sham control group mice were given sham operation.And the other two groups were given myocardial infarct operation.After one week of operation,sham control group mice were intraperitoneally injected with NS,while the other two groups were injected with LPS.After 30min,the microcirculation flow velocity of cremaster was observed.Then the different means of intervention were given to the three group mice.The microcirculation flow velocity at different time points and white blood cells adhesion were recorded.At last,the relative expression of endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) mRNA were measured.Results Compared with those of sham control group,the microcirculation flow velocity of MI + LPS group was decreased significantly (P ＜0.05)and the white blood cells adhered to endothelial cells of MI + LPS group was increased (P ＜0.05).Compared with those of MI + LPS group,the microcirculation flow velocity of STDP group increased significantly (P ＜0.05)and the white blood cells adhered to endothelial cells of STDP group decreased (P ＜ 0.05),the relative expression of eNOS and nNOS mRNA of STDP group increased significantly (P ＜0.05).Conclusion STDP can improve the microcirculation in mice.The more produced endogenous NO and the decreased white blood cells adhered to endothelial cells might be the mechanism.
Keywords：myocardial infarctionmicrocirculation dysfunctionShexiang Tongxin dropping pilllipopolysaccharides
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2969-2972 )