Experimental Study on Panax notoginseng Saponins Attenuates Isoproterenol-induced Myocardial Injury

Yang Qinbo; Cui Jingang; Wang Peiwei; Ding Liliqiang; Chen Yu; Zhang Teng

doi:10.3969/j.issn.1672-1349.2017.20.006

Abstract：Objective To investigate the effect of panax notoginseng saponins (PNS) on isoproterenal (ISO)-induced myocardial injury and explore the mechanism of PNS anti myocardial injury.Methods C57 mice were divided into vehicle control (VC) group,ISO induced myocardial injury (ISO) group,and PNS treatment (PNS) group.All mice were intraperitoneally injected vehicle control,ISO,ISO,and PNS separately for 7 days.Heart was utilized for HE stain and massion trichrome stain were performed in heart to access the therapeutic effect of PNS on ISO-induced myocardial injury.Further,real time-PCR was carried out to detect the expression of miR-1 and miR-222 in heart,and Met was probed in heart using immunohistochemical staining to discover the role of miR-1/MET/miR-222 in the anti-myocardial injury effect of PNS.Results The pathological staining showed that ISO induced myocardial injuries including necrotic degeneration,granulation as well as inflammatory cell infiltration,and collagen accumulation which was invariably observed in ISO challenged mice.Whereas,slight necrosis,sporadic infiltration of inflammatory cells and accumulation of collagen were observed in PNS treated mice.Down regulated expression of miR-1 and up regulated expression of miR-222 were demonstrated in heart from ISO induced myocardial injury mice compared to that from vehicle control mice.While expression of miR-1 and miR-222 were rescued in heart from PNS treated mice.The level of MET was increased in heart from ISO challenged mice and it focused in the area of injury.But the positive stain of MET in heart from PNS treated mice was significantly less than that from ISO challenged mice.Conclusion PNS has a dominantly therapic effect on ISO induced myocardial injury.MiR-1/MET/miR-222 axis may play an important role in the pathological process of myocardial injury induced by ISO and anti-myocardial injury effect of PNS.