The Expression of RECK in Brain Tissue after Diabetic Cerebral Ischemic Infarction and Its Relation with Matrix Metalloproteinase-9
Shangguan Shina
Zhu Meijia
Abstract：Objective To explore the involvement of reversion inducing cysteine rich protein with Kazal motifs (RECK) and matrix metalloproteinase-9 (MMP-9) in diabetic cerebral ischemia.Methods One hundred and ten wistar rats were randomly divided into simulate operation group,cerebral ischemia group(CI group) and diabetic cerebral ischemia group(DM + CI group).The diabetic rat models were established by high fat,saccharose diets and streptozotion injections.The middle cerebral artery occlusion models were made by improved zealong's method.The expressions of RECK and MMP-9 were examined by immunohistochemistry staining.Results RECK and MMP-9 located mainly in the cytoplasm of neurons,neuroglia cells and etc in the ischemic penumbra zones.RECK distribution significantly decreased in both ischemic groups (P ＜ 0.01).At every reperfusion point,less RECK(+)cells in DM + CI group were detected than CI group (P ＜0.05).RECK decreased at 3 hours,minimum at 24 hours,then gradually increased after 3 days in both ischemic groups.MMP-9 significantly increased in both ischemic groups (P ＜ 0.05).MMP-9 increased significantly more than that in CI group (P ＜0.05),peaked at 24 hours.Negative correlation of MMP-9 in DM + CI group and RECK were detected at 3 hours to 24 hours after reperfusion.Conclusion The reduction of RECK possibly contribute to the increase of MMP-9,which may be one reason for infarction injuries and terrible results in cerebral ischemia of diabetic patients.
Keywords：diabetes mellituscerebral ischemic infarctionreversion inducing cysteine rich protein with Kazal motifsmatrix metalloproteinase-9vascular remodling
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2393-2395 )