Effects of D-galactose, Sodium Nitrite and Alchlor on Cognitive Competence in Mice

Liu Meixun; Sun Tianmin

doi:10.3969/j.issn.1672-1349.2017.18.009

Abstract：Objective To investigate the effect of D-galactose,sodium nitrite,and alchlor on cognitive competence in mice.Methods The health mice were selected and divided into Alzheimer's disease (AD) model group and normal group.Mice in model group were injected with alchlor,D-galactose and sodium nitrite for three consecutive months.Mice in normal group were injected with normal saline of the same volume.The behavior,anti-oxidative index and morphological staining pattern were observed.Results The incubation period and the time when the original platform position passed through for the first time in AD model group were significantly longer than that in normal group (P ＜ 0.01).The activity time in the original platform position the former were significantly shorter in AD model group than that in normal group (P ＜0.01).The activities of SOD,GSH-Px decreased in AD model group,which was lower than that in normal group (P ＜0.01).MDA concentration rises in AD model group,which was higher than that in normal group (P ＜0.01).Congo red stainning method showed that many Aβ spots could be seen in hippocampal CA2 sector in AD model group,while no Aβ spot was observed in normal group.Improved amine silver staining method showed that few and irregular pyramidal cells could be found in hippocampal CA2 sector in AD model group.There was thickened black filament around the nucleus and it showed a forma of small patch in some area,and age pigment could be seen outside the neuron.However,the tissue structure of hippocampal CA2 sector of the normal group was clear.Conclusion AD pathological changes are partially modeled by the combined and chronic effect of D-galactose,sodium nitrite and alchlor in mice,which is proved to be an ideal method to establish AD animal model.