Cardioprotective Effects of Hesperetin Against ISO-induced Heart Failure in Rats
Cao Jing
Lv Jiyuan
Zhang Mingsheng
Shi Ruizan
Feng Qiaoai
Abstract：Objective To explore the protective effects and possible mechanisms of hesperetin against isoproterenol (ISO)-induced heart failure (HF) in rats.Methods Forty Sprague-Dawley (SD) rats were randomly divided into four groups:control group(group C),hesperetin group (group H),isoproterenol group (group Ⅰ)and H+ Ⅰ group (group T).Rats in group Ⅰ and group T were subcutaneously injected with ISO (150 mg/kg) once a day for 2 consecutive days.The model was successfully established in heart failure was determined by echocardiography after 2 weeks.Hesperetin was administered at the dosage of 100 mg/kg,po by gavage for a period of 10 days in group H and group T.Cardiac function of all rats including left ventricular end-diastolic diameter (LVEDD),left ventricular end-systolic dimension (LVESD),left ventricular ejection fractions (EF),left ventricular fractional shortening (FS) was assessed by echocardiography.Collecting serum of all rats,malondialdehyde (MDA),superoxide dismutase (SOD),total antioxidant capacity (TAOC) were detected by thiobarbiturieacid colorimetry,WST-1 method and colorimetry method respectively.Results There was not obvious changes of cardiac function in group C and group H.ISO inhibited the cardiac function of rats significantly including LVESD,EF,as well as FS,compared to group C(P ＜0.05).LVESD was increased while EF and FS were decreased.The level of MDA in group Ⅰ was significantly higher (P ＜0.05) while the levels of SOD and TAOC were significantly lower than group C (P ＜0.05).Hesperetin attenuated the effect of heart failure induced by ISO.Cardiac function of rats including LVESD,EF,as well as FS was improved in group T compared to group Ⅰ (P ＜0.05).LVESD was declined,EF and FS were ascended.The level of MDA was significantly lower,the levels of SOD,TAOC in group T were significantly higher than that in group Ⅰ (P ＜0.05).Conclusion Hesperetin can attenuate ISO-induced heart failure in rats.Its possible mechanisms may be associated with regulating oxidative stress.
Keywords：heart failurehesperetinisoproterenoloxidative stressrat
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2241-2244 )