Effects of Edaravone Injection on Serum S100B Protein in Neonatal Rats with Hyperbilirubinemia Induced Brain Injury
Shen Yajie
Yin Huaiqing
Cao Fangfang
Jia Xiaojun
Abstract：Objective To explore the effects of edaravone injection on serum S100B protein in neonatal rats with hyperbilirubinemia induced brain injury.Methods Ninety-six seven-day-old Wistar Sprague-Dawley (SD) rats were randomly assigned into three groups:normal control group (group A),hyperbilirubinemia group (group B),and edaravone injection group (group C).Then each group was divided into 4 subgroups based on the time of execution.The rats in group B and group C were injected intraperitoneally with bilirubin.The rats in group A were injected intraperitoneally with normal saline once.After modeling,rats in group C were given intraperitoneal injection of edaravone injection.The serum and brain tissue were taken out at different time points.The pathological changes of brain tissue were observed by HE staining.The concentration of serum S100B protein was measured by Elisa method.Results After modeling,neurological abnormalities were observed in group B and group C.The symptoms of rats in group C had significantly improved compared with group B at each time point.After 24 hours of modeling,the edema,degeneration and inflammatory infiltration of brain tissue in group B and group C were aggravated with the prolongation of time.The changes of brain tissue in group C were lower than those in group B at each time point.The structure of brain tissue in group A was normal.The concentration of S100B protein in group B was higher than that in group A and group C,and positively correlated with time (P<0.05).The concentration of serum S100B protein in group C was lower than that in group B (P<0.05).Conclusion After intervention with edaravone injection,the changes of brain tissue in the hyperbilirubinemia rats at different time points were reduced to different degrees.The concentration of S100B protein was significantly decreased at different time points.The results indicated that edaravone injection could reduce the damage of excess bilirubin on central nervous system,and play a role in protecting neurons.
Keywords：hyperbilirubinemiabrain injuryedaravone injectionS100B protein
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2116-2119 )