Effects of Zhichan Decoction on the Phosphorylation of DARPP-32 and Dyskinesia in Parkinsonian Rats
Huang Ningjing
Lu Xiaoqing
Abstract：Objective To study the effects of Zhichan decoction (ZCD) on the phosphorylation of the dopamine and cAMP-regulated phosphoprotein of 32 kDa (DARPP-32) at Thr34 in striatum of Parkinsonian rats with abnormal involuntary movements (AIM).Methods Six unilatera1 hydroxydopamine-lesioned rat,the mode1 of Parkinson disease (PD),were treated with levodopa/benserazide in order to establish the rats models with levodopa-induced dyskinesia (LID).LID rats were randomly divided into 5 groups: LID model groups treated with saline,levodopa group and ZCD low doses group,ZCD medium doses group and ZCD high doses group,respectively.Five rats with sham-operation were served as controls.Rats were then sacrificed,and immunohistochemica1 techniques was used to measure the expression of phosphorylated DARPP-32 at Thr34 in striatum.Results Immunohistochemistry showed that the expression of phosphorylated DARPP-32 at Thr34 in striatum in LID model group was higher than that in control group (P<0.01).The expressions of phosphorylated DARPP-32 at Thr34 in striatum in ZCD groups were lower than that in levodopa group (P<0.01).The expression of phosphorylated DARPP-32 at Thr34 in striatum in ZCD medium doses group was lower than that in ZCD low doses group (P<0.05).Conclusion ZCD can down-regulate the expression of phosphorylated DARPP-32 at Thr34 in striatum in Parkinsonian rats with LID.
Keywords：Parkinson's diseaseZhichan decoctiondyskinesiadopamine and cAMP-regulated phosphoprotein of 32 kDa
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2110-2113 )