Effects of Carbamylated Erythropoietin on the Apoptosis and Cleaved Caspase-3 Protein in Rat with Chronic Heart Failure
Huang Zhaoqi
Wu Jinlei
Xu Wei
Chen Yongquan
Xie Wenjie
Chen Ximing
Chen Shengqiang
Abstract：Objective To observe the effects of cardiomyocytes carbamylated erythropoietin (CEPO) on the cardiac function,the apoptosis of cardiomyocytes,protein cleaved cysteine-containing aspartate-specific proteases (cleaved Caspase-3) expression in rats with chronic heart failure(CHF).Methods Firstly,30 male adult SD rats were randomly divided into three groups,sham-operated (Sham) group (n=10),CEPO group (n=10) and control (Control) group (n=10).CHF model was built by abdominal aortic coarctation.After postoperative eight weeks,rats in CEPO group received 50 μg / kg CEPO by intraperitoneal injection 3 times / week for 4 weeks.The rats in Sham group and Control group received equal normal saline.Respectively,after 8 weeks and 12 weeks,cardiac function was observed with echocardiography.After 12 weeks,all rats were sacrificed,cell morphology was observed by HE staining.The myocardial apoptosis and calculated apoptosis index (AI);was observed Myocardial cleaved Caspase-3 protein expression was observed by western blot.Results Echocardiography showed CHF rats had ventricular hypertrophy and heart failure in 8 weeks.CEPO intervention after 4 weeks compared with Control group,left ventricular ejection fraction (LVEF) was significantly higher (P<0.05),systolic interventricular septum thickness (IVSs),end-systolic left ventricular posterior wall thickness (LVPWs),diastolic interventricular septum thickness (IVSd), left ventricular end-diastolic wall thickness (LVPWd) was significantly lower (P<0.05).AI in CEPO group was significantly lower than that in Control group[(23.87±1.45)% vs(30.15±0.67)%,P<0.05].The cleaved Caspase-3 OD values significantly decreased in CEPO group (0.489 1±0.029 1) with Control group (P<0.01).Conclusion CEPO can improve heart function in rats with CHF the cardiomyocyte apoptosis,by down-ragulating the express of cleaved Caspase-3 protein.
Keywords：heart failurecarbamylated erythropoietincardiac apoptosiscleaved Caspase-3 protein
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1974-1978 )