Tetramethylpyrazine Ameliorates High Blood Glucose Fluctuations Induced-human Umbilical Vein Endothelial Cell Injury by Regulating PKCβ1 Expression
Wang Jingshang
Huang Ye
Yin Huijun
Sun Mingyue
Shi Ying
Abstract：Objective To investigate the effects of Tetramethylpyrazine (TMP) on human umbilical vein endothelial cells (HUVECs) injury induced by high blood glucose fluctuations and the PKCβ1 expression.Methods HUVECs were incubated for 8 days in media containing different glucose concentrations:5.56 mmol/L (normal glucose,N),25 mmol/L (constant high glucose,W),or a daily alternating 5.56 or 25 mmol/L glucose (fluctuant high glucose,B).LY333531 (200 nmol/L) was pretreated on W group (WL) and B group (BL),respectively.Meanwhile,B group were treated with tetramethylpyrazine hydrochloride (500 μmol/L) and metformin hydrochloride (1 mmol/L),respectively,which were called as TMP group and MH group.Cell apoptosis was measured by flow cytometry.The concentrations of tumor necrosis factor-alpha (TNF-α),soluble intercellular adhesion molecule-1 (sICAM-1),AOPP and total antioxidant capacity (T-AOC) in the cell culture supernatant were measured by ELISA method.The expression of protein kinase C (PKC) β1 in HUVECs was measured by Western blot.Results In comparison with N group,cell apoptosis rate (mainly late apoptosis),levels of TNF-α,sICAM-1 and AOPP,the expression of PKCβ1 in W group and B group were all significantly increased (P<0.05 or P<0.01),whereas T-AOC was significantly decreased (P<0.01),all especially in B group (P<0.05 or P<0.01).Pretreated with LY333531,cell apoptosis rate,levels of TNF-α,sICAM-1 and AOPP,as well as the expression of PKCβ1,were all significantly decreased (P<0.05 or P<0.01),meanwhile T-AOC was significantly increased (P<0.05 or P<0.01).TMP and MH manifested the similar protective effects as LY333531.Conclusion High blood glucose can induce HUVEC injury obviously,which is closely related to PKCβ1 over-expression.Tetramethylpyrazine can protect HUVECs suffering fluctuant high glucose obviously,and the protective mechanism of which is closely related to its effects of relieving vessel stress,alleviating inflammatory reaction and inhibitory effect on the expression of PKCβ1.
Keywords：human umbilical vein endothelial cells injurytetramethylpyrazinehigh blood glucose fluctuationsprotein kinase Cβ1tumor necrosis factor-alphasoluble intercellular adhesion molecule-1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1969-1973 )