Effects of Exendin-4 on Amyloid β-protein Induced Impairments of Learning Memory and Intracellular Calcium Concentration of Hippocampal Neuron in Rats
Pan Yanfang
Fang Yan
Ying Xiaoping
Abstract：Objective To explore the effects of exendin-4 on amyloid β-protein (Aβ) 1-42-induced deficits in learning memory and intracellular calcium concentration of hippocampal neuron in rats.Methods Forty male Sprague-Dawley (SD) rats were randomly divided into four groups (n=10,each group):control, Aβ1-42 , Exendin-4, Exendin-4+Aβ1-42.After bilateral hippocampal injection of Aβ1-42 and exendin-4 rats,Morris water maze test was used to test the ability of spatial learning and memory,and laser-scanning confocal calcium imaging technique was used to test the [Ca2+]i in primary cultured rat hippocampal neurons.Results Hippocampal injection of Aβ1-42 alone impaired spatial learning and memory of rats (P<0.05).Exendin-4 did not affect learning and memory of rats,but alleviated Aβ1-42-induced spatial learning and memory deficits,compared with Aβ1-42 alone group (P>0.05).Pretreatment with exendin-4 suppressed Aβ1-42-induced elevate on in intracellular calcium concentration [Ca2+]i.(P<0.05).Conclusion These findings indicate that systemic pretreatment with exendin-4 can effectively prevent the impairment in spatial learning and memory of rats induced by neurotoxic Aβ1-42, and the underlying protective mechanism of exendin-4 may be involved in regulation of calcium homeostasis.
Keywords：Alzheimer's diseaseexendin-4amyloid β-proteinspatial learning and memoryintracellular calcium concentration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1834-1837 )