Effect of Strengthing Qi and Activating Blood Chinese Medicine on Myocardial Mitochondrial Biogenesis Related Protein in Rats after Acute Myocardial Infarction
Yan Huijing
Liu Jiangang
Dong Ruihong
Zhang Dawu
Wang Chenglong
Abstract：Objective To observe the effect of strengthing qi and activating blood Chinese medicine Panax quinquefolius saponin (PQS) and refined Xuefu Capsules on AMPK activated-mitochondrial biogenesis protein in rats myocardium after acute myocardial infarction (AMI).Methods Sixty male Sprague-Dawley (SD) rats, fed with high-fat diet for 28 d, after anesthetized with 4% chloral hydrate, 26 of them were made as AMI model successfully by descending artery ligation.And all of AMI model rats were randomly divided into model group, strengthing qi and activating blood Chinese medicine group, for 13 rats in each group.And set 13 rats as sham group (only puncture,without ligation).The rats of strengthing qi and activating blood Chinese medicine group were gavaged with the strengthing qi and activating blood Chinese medicine, with PQS was given 162 mg/(kg·d),refined Xuefu capsules was given 3.6 mg/(kg·d) since the next day of surgery.At the same time, the rats of model group and sham group are gavaged with equal amount of distilled water.After 28 d, the rats were anesthetizedto measure left ventricular end-systolic inner diameter(LVDs), left ventricular end-diastolic diameter(LVDd), ejection fraction(EF)by Ultrasonic echocardiography.When finished, the chest was opened, and the hearts was removed for histopathology testing and the gene and protein of AMP-activated protein kinase α2 (AMPKα2), Peroxisome proliferator-activated receptor-γ co-activator1α (PGC1α) in rats myocardium testing.Results Echocardiography showed that compared with sham group, LVDs and LVDd value in model group significantly increased (P<0.05) and EF values decreased significantly(P<0.05).Compared with the model group, the LVDs and LVDd value in strengthing qi and activating blood Chinese medicine group reduced obviously (P<0.05) and EF value in strengthing qi and activating blood Chinese medicine group increased obviously (P<0.05).RT-PCR showed that compared with sham group, AMPKα2 and PGC1α genes of myocardial tissues in model group down-regulated (P<0.05).Compared with the model group, AMPKα2 and PGC1α genes of myocardial tissues in strengthing qi and activating blood Chinese medicine group up-regulated (P<0.05).Western Blot showed that compared with sham group, protein expression of AMPKα2 and PGC1α of myocardial tissues in model group down-regulated (P<0.05).Compared with the model group, protein expression of AMPKα2 and PGC1α of myocardial tissues in strengthing qi and activating blood Chinese medicine group up-regulated (P<0.05).Conclusion Strengthing qi and activating blood Chinese medicine can inhibit left ventricular remodeling, and improve left ventricular function after AMI.The role of protection may related to the promotion of mitochondrial biogenesis protein (AMPKα2 and PGC1α) expression in myocardium after AMI.
Keywords：acute myocardial infarctionsupplementing qi and activating bloodmodel ratsAMP-activated protein kinaseperoxisome proliferator-activated receptor-γ co-activator 1α
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1829-1833 )