The Effects of Xuebijing Injection on Cardiac Function and Endothelial Function in Rats with Sepsis
He Jianzhuo
Yin Xin
Ma Shiyu
Guo Liheng
Zhang Minzhou
Abstract：Objective To observe the protective effects of Xuebijing injection (XBJ) on the myocardium and serum nuclear factor-κB (NF-κB) expressions, endothelin-1 (ET-1), von Willebrand factor (vWF) and endothelial nitric oxide synthase (eNOS) in rats with sepsis,and to investigate the protection of cardiac dysfunction.Methods Forty-two male Sprage-Dawely (SD) rats were divided randomLy into sham group (n=6),CLP group (n=18) and XBJ group (n=18).The animal models of sepsis were induced by cecal ligation and puncture (CLP) and XBJ was given by intraperitoneal injection.Heart rate, cardiac function such as left ventricular ejection fraction (LVEF), left ventricular end-diastolic diameter (LVESD), left ventricular end diastolic diameter (LVEDD), intact ventricular septum (IVS) activity were observed.The histopathological changes were observed by Hematoxylin-Eosin (HE) staining.The levels of serum ET-1, vWF, eNOS and NF-κB were measured by enzyme-linked immunosorbent assay (ELISA).The expression of NF-κB in cardiac tissue was detected by immunohistochemical staining.Results After 24 hours of modeling, compared with sham group, the heart rate,LVESD and LVEDD increased while IVS activities degree decreased in CLP group (P<0.05).The heart rate increased while LVEF decreased in XBJ group (P<0.05).Compared with 24 hours,the heart rate decreased in 48 hours in CLP group (P<0.05).Compared with CLP group, the heart rate,LVESD decreased while LVEF and IVS activity increased in XBJ group in 24 hours and 48 hours (P<0.05).After 24 hours of modeling, compared with sham group,the levels of serum ET-1 and vWF increased while eNOS level decreased in CLP group(P<0.05).The levels of serum ET-1 and vWF in XBJ group were lower than that in CLP group(P<0.05) while eNOS level in XBJ group was higher than that in CLP group(P<0.05).After 48 hours of modeling, ET-1 level in XBJ group was lower than that in CLP group(P<0.05).After 24 hours of modeling, compared with sham group, serum NF-κB level increased in CLP and XBJ group (P<0.05) ,which was lower in XBJ group than that in CLP group(P<0.05).The rate of NF-κB positive cells in CLP group was higher than that in sham group(P<0.05),whilewas lower in XBJ group than that in CLP group (P<0.05).Conclusion XBJ could improve cardiac and endothelial function in CLP rats,which might be through the intervention of NF-κB pathway,decreasing the expression of NF-κB,thereby increasing the expression of eNOS,reducing the endothelial damage.
Keywords：sepsisXuebijing injectionratsmyocardiumnuclear factor-κBendothelium function
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1710-1715 )