Effects of Naloxone,Epinephrine and Vasopressin on Cytokines in Resuscitation Rats
Tang Tianjun
Wu Jun
Huang Zhongrong
Zhang Jihong
Abstract：Objective To investigate the effects of naloxone,epinephrine and vasopressin on cytokines in resuscitation rats,and to explore the possible mechanism.Methods One hundred healthy wistar rats,weight in 280 g to 320 g,were randomly divided into five groups:sham operation group (group A,n=20), epinephrine group (group B,n=20),epinephrine plus vasopressin group (group C,n=20),epinephrine,vasopressin plus naloxone group (group D,n=20) and control group (group E, n=20).Rats were treated with anesthesia and tracheal intubation in group A.Rats in group B, groupC, group D and group E were successfully established to cardiac arrest model,adrenaline 0.2 mg/kg,vasopressin 0.8 U/kg and naloxone 0.4 mg/kg and normal saline in tail vein were injected, respectively.The changes of mean arterial pressure (MAP) in 30 min after spontaneous circulation recovery in group B,group C and group D were recorded.The levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-10 were detected by immunohistochemical method.Results Compared with group E,there were no significant difference in asphyxia time between group B, group C and group D (P>0.05).Compared with group C and group B,there was a significant difference in the success rate of spontaneous circulation in group D (P<0.05).When 30 min after return of spontaneous circulation (ROSC), MAP had an upward trend in grup B,group C,group D.Compared with group B, MAP increased more significantly in group C and group D (P<0.05).At the same time, there was a significant difference in the levels of TNF-α,IL-6 and IL-10 between group E and group B,or group C,or group D (P<0.05).Conclusion During cardiopulmonary resuscitation (CPR),naloxone,epinephrine and vasopressin can significantly increase the rate of spontaneous circulation restoration.At the same time, arterial pressure maintained at a relatively high level in rats after the recovery.Naloxone,epinephrine and vasopressin can reduce the levels of serum proinflammatory cytokines,improve the levels of serum anti inflammatory cytokines in rats.
Keywords：cardiopulmonary resuscitationnaloxoneepinephrinevasopressintumor necrosis factor alpha
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1453-1456 )