Effects of Curcumin on Ventricular Remodeling in Diabetic Cardiomyopathy Rats and Its Mechanism

Xiong Xuesong; Qi Zhonglin

doi:10.3969/j.issn.1672-1349.2017.12.007

Abstract：Objective To explore the effects of curcumin on blood glucose and ventricular remodeling in diabetic cardiomyopathy rats (DCM).Methods Forty male wistar rats were randomly divided into control group (n=10), model group (n=10) , curcumin group (n=10),and positive control group(n=10) after one week of adaptive feeding.The high-fat high-calorie diet plus single chain of 40 mg/kg streptozotocin with intraperitoneal injection were used to establish animal model of DCM, rats in curcumin group and positive control group rats were given intragastric administration of 40 mg/(kg·d) naringin;6% ethanol and 6% polyethylene glycol mixture rats in control group and model group intraperitoneal, administrated 8 weeks.Rats in each group were measured for blood glucose changes before and after drug administration;after 8 weeks of administration, the heart function indexes were measured by high frequency echocardiography;then the rats were sacrificed.The myocardial tissue was taken for HE staining,Masson for collagen volume fraction analysis.Apoptosis of myocardial cell were detected by TUNEL.The expressions of matrix metalloproteinase (MMP)-9 and MMP-2 were detected by ELISA in myocardium of rats, and image analysis by HemI software.The expression of GRP78, IRE1, JNK and p-JNK in rat myocardium were detected by western blot.Results Compared with the model group, the rats in curcumin group after 8 weeks of administration, the blood glucose decreased significantly (P>0.05).The echocardiography showed that left ventricular remodeling indicators were significantly improved(P<0.05).The myocardial fibrosis integral and MMP-9 and MMP-2 expressions were significantly decreased in curcumin group (P<0.05).The expressions of GRP78, IRE1, JNK,p-JNK and cell apoptosis were significantly reduced in myocardial tissue of the rats in curcumin group(P<0.05).Conclusion Curcumin can decrease the blood glucose of DCM rats, reduce myocardial fibrosis, anti-myocardial cell apoptosis,inhibit ventricular remodeling in DCM rats.