The Cardioprotection of Globular Adiponectin on Diabetic Cardiomyopathy in Rats
Wu Hongru
Li Xing
Gao Xiaofang
Han Xuehong
Liu Jing
Abstract：Objective To observe the effect of globular adiponectin (gAd) on diabetic cardiomyopathy (DCM) and to explore its possible mechanism.Methods Thirty male Sprague-Dawley (SD) rats were divided into normal control group (group A),and 20 rats were treated with diabetic cardiomyopathy model,the rats were randomly divided into positive control group (group B) and spherical adiponectin group (group C).Rats in group C were given globular adiponectin daily.After 8 weeks of treatment,the morphology of myocardium was observed by hematoxylin-eosin (HE) staining.The expression of type Ⅰ collagen,transforming growth factor-β1 (TGF-β1),Smad 3 and Smad 7 in myocardium of rats were detected by immunohistochemistry.The apoptosis of myocardium was detected by TUNEL.Results HE staining showed that the arrangement of myocardial cells in group B was disturbed,and the arrangement of cardiomyocytes in group C was more neat than group B.The expressions of type Ⅰ collagen,TGF-β1 and Smad 3 protein in group B was higher than that in group A and group C (P<0.05).The expression of Smad 7 protein in group B was lower than that of group A and group C (P<0.05).TUNEL results showed that the number of apoptotic cells in group B was higher than that of group A and group C (P<0.05).Conclusion Globular adiponectin may protect cardiomyocytes by alleviating myocardial interstitial fibrosis and cardiomyocyte apoptosis.
Keywords：diabetic cardiomyopathyglobular adiponectintransforming growth factor-β1fibrosisapoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1319-1324 )