The Influence of Tanyuqing Formula on the Blood Pressure and the Arterial Remodeling in Spontaneously Hypertensive Rat

Liu Chun; Zhou Lihua

doi:10.3969/j.issn.1672-1349.2017.07.008

Abstract：Objective To observe the effects of Tanyuqing formula(TYQF) on the blood pressure,the arterial remodeling of the thoracic aorta and the second branch of superior mesenteric artery in spontaneously hypertensive rat (SHR),and to study the mechanism of antihypertensive effect of TYQF.Methods Forty male SHR were assigned to 5 groups randomly:SHR control group,SHR+Fosinopril,SHR+ TYQF high dose group,SHR+ TYQF medium dose group,SHR+ TYQF low dose group.Each group consisted of 8 animals.8 male WKY rats (11 week-old) were used as normal control group.The rats in SHR+ TYQF high dose group,medium and low dose group were given TYQF contained crude drug of 23.24 g/kg,11.62 g/kg,5.81 g/kg respectively.The rats in SHR+Fosinopril were given suspl of fosinopril of 0.9 mg/kg.The rats in SHR and WKY control group were given the distilled water of the same volume.Drugs were given for 8 weeks constantly.Before treated and after every two weeks,the blood pressure and heart rate of every rat was measured.Thoracic aorta and the second branch of superior mesenteric artery were harvested at 20 week-old.After hematoxylin and eosin staining,the external diameter (ED),lumen diameter (LD),medial thickness (MT),medial thickness/lumen diameter (MT/LD) of aorta and the second branch of superior mesenteric artery were measured.After Masson's trichrome staining,the collagen volume fraction (CVF) of tunica media of aorta and the second branch of superior mesenteric artery were measured.Results There was a significant positive correlation between the blood pressure (P＜0.001).The blood pressure was increased compared with that of WKY (P＜0.001).The blood pressure in SHR+ TYQF high and medium dose group were reduced compared with that of SHR (P＜0.001).The values of ED,LD,MT,MT/LD of thoracic aorta of SHR were increased compared with that of WKY (P＜0.05 or P＜0.001).The values of ED,LD in SHR+ TYQF high dose group were reduced compared with that of SHR (P＜0.05 or P＜0.01).The values of MT in SHR+ TYQF high dose group were reduced compared with that of SHR,but there was no statistic significance.The values of MT/LD of SHR were not significantly different from that of three groups of SHR+ TYQF.The values of CVF in SHR were increased compared with that of WKY (P＜0.001).The values of CVF of thoracic aorta SHR+ TYQF high and medium dose group were reduced compared with that of SHR (P＜0.05 or P＜0.001).The values of ED of the second branch of superior mesenteric artery of SHR were not significantly different from that of WKY.The values of LD in SHR were reduced compared with that of WKY (P＜0.001).The values of MT,MT/LD in SHR were increased compared with that of WKY (P＜0.05,P＜0.01,P＜0.001).The values of LD in SHR+ TYQP high dose group was increased compared with that of SHR,SHR+ TYQPF medium and low dose group(P＜0.05,P＜0.01,P＜0.001).The values of MT/LD in SHR+ TYQF high dose group was reduced compared with that of SHR,SHR+ TYQF medium and low dose group(P＜0.05,P＜0.01,P＜0.001).Other index of SHR was not significantly different from that of three groups of SHR+ TYQF.The values of CVF of tunica media of the second branch of superior mesenteric artery of SHR were increased compared with that of WKY (P＜0.05).The values of SHR+ TYQF high and medium dose group were reduced compared with that of SHR (P＜0.05).The values of SHR+ TYQF high and medium dose group were reduced compared with that of SHR+ TYQF low dose group(P＜0.01).Conclusion TYQF could decrease the blood pressure and heart rate in SHR.TYQF could improve the remodeling of thoracic aorta and the second branch of superior mesenteric artery in SHR.