Effect of Tongluo Decoction on the Coronary Microembolization in Rats

Zhang Shuqin; Zhang Zhenguo; Li Xuefeng; Zhu Yan; Zhao Junnan; Zhou Jing; Wang Ying

doi:10.3969/j.issn.1672-1349.2017.05.010

Abstract：Objective To explore the effect and its mechanism of Tongluo decoction (TLD) on the coronary microembolization in rats.Methods Twenty-six male SD rats were divided into TLD group(n=10),model group(n=10),control group(n=6).The rats of TLD group and model group were injected 0.3 mL autologous micro thrombus particles by intervention operation via right common carotid artery for building coronary microembolization model.Postoperation 24 hours,rats of TLD group were given intervention drug by gavage once daily for 9 days.The rats of model group and control group were given sterile water by gavage once daily for 9 days.To observe the number of vascular embolization and the area of myocardial ischemia,taking the apex of left ventricular with paraffin section through pathological staining.The level of serum cTnI was tested by ELISA.The expression of tumor necrosis factor-α (TNF-α) and interleukin(IL)-1 β in myocardium were tested by immunohistochemical.Results HE staining showed that the number of coronary microembolization in model group was obviously higher than that in control group (P<0.05).It showed that coronary microembolization model of rats were built successfully.There was no difference in the number of coronary microembolization between TLD group and model group (P>0.05).The emboli of coronary microembolization in TLD group were becoming smaller than ever and obviously organized.HBFP staining showed that the positive staining area in model group was significantly bigger than that in TLD group and control group (P<0.05).Compared with model group,serum cTnI level in TLD group was decreased (P<0.05).The expression of TNF-α and IL-1β in myocardium in TLD group was obviously lower than that in model group (P<0.05).Conclusion TLD could significantly inhibit the pathological change of coronary microembolization,reduce the injury of myocardial cell,inhibit the expression of inflammatory cytokines and promote the microemboli vascular recanalization.