Effects of Shudi Pingchan Granule on Behavior and α-synuclein Expression in Rats with Parkinson's Disease
Ye Qing
Wang Kai
Yuan Xiaolei
He Jing
Zhou Jie
Yuan Canxing
Abstract：Objective To observe the effect of Shudi Pingchan granule (SPG)on behavior andα-synuclein (α-syn)expression in substantia nigra in rats with Parkinson's disease (PD). Methods The rat model of hemiparkinsonian was established by injecting 6-hydroxydopamine (6-OHDA)into substantia nigra of right brain. Then,the rats were divided into normal control group,PD group, levodopa (LD)group,and LD plus SPG group. After 4 weeks of treatment,the number of turns,total duration of turning and abnormal involuntary movement (AIM)scores were observed. The gene expression ofα-synuclein was detected by real-time polymerase chain reaction (RT-PCR). The protein expression ofα-syn and TH was detected by immunohistochemistry. Results The number of turns in PD group had no significant difference compared with that before treatment(P>0.05),and total duration of turning short-ened (P<0.05).The total duration of turning in LD group on the 7th and 14th day were lower than those in PD group in the same time,but they were higher than those in PD group on the 28th day(P<0.05). The number of turns and total duration of turning in LD+TCM group was significantly lower than those of PD group since the 14th day(P<0.05). The AIMs of LD group increased graduallysince the 14th day(P<0.01). The scores of AIM in LD plus SPG group had no difference compared with that before treatment(P>0.05). Compared with normal control group,α-syn mRNA expression increased significantly in PD group.Compared with PD group,α-syn mRNA significantly increased in LD group. The expression ofα-syn mRNA in LD plus SPG group decreased obviously compared with LD group (P<0.05). Compared with the normal control group,α-syn expression increased and TH expression reduced in PD group. Compared with PD group,α-syn expression increased and TH expression decreased in LD group. Compared with LD group,α-syn expression decreased and TH expression increased in LD plus SPG group (P<0.01).Conclusion Long-term use of LD can cause over expression ofα-syn and further aggravatenerve damage in midbrain nigra,which makes the behavioral symptoms worse. SPG and LD treating PD has effect on apparente synergia and attenuation. SPG can effectively alleviate the symptoms of PD by inhibiting over-expression ofα-syn,reducing the damage of dopaminergic cells.
Keywords：Parkinson's diseaseShudi Pingchan granulebehaviorα-synucleinapparente synergia and attenuation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 419-424 )