The Influence of Ligustrazine on Cardiac Fibroblasts Fibrosis Induced by Angiotensin Ⅱin Rats
Chang Yuyin
Lu Ming
Lu Tailing
Ding Jie
Abstract：Objective To observe the effect of ligustrazine on angiotensinⅡ(AngⅡ) induced cardiac fibroblasts(CFs)fibrosis and its mechanisms.Methods The CFs of neonatal Sprague Dawley (SD)rats were isolated with the method of trypsin digestion and dif-ferential anchoring velocity,then cultured in vitro.The generation 2 4 of CFs were used for the experiment and randomly divided into 5 groups which were control group cultured without AngⅡ or ligustrazine(group A),AngⅡ group cultured with AngⅡ 10-6 mol/L (group B),and ligustrazine groups(group C,group D,group E)cultured with AngⅡ 10-6 mol/L and ligustrazine 5,10,20 mg/L,respec-tively.CFs were cultured for 48 h and the smooth muscle alpha actin (α SMA),beta isoform of calcineurin (CnAβ)mRNA expres-sion were examined by reverse transcription polymerase chain reaction (RT PCR),theα SMA,nuclear factor of activated T lym-phocyte 3 (NFAT3)protein expression by western blot analyses.Results Compared with group A,the expressions ofα SMA,CnAβmRNA andα SMA,NFAT3 protein were upregulated in group B(al P<0.05).Compared with group B,the expressions ofα SMA, CnAβmRNA andα SMA,NFAT3 protein in group D and group E were obviously decreased(al P<0.05).Conclusion Within a giv-en concentration range,ligustrazine can inhibit the expression ofα SMA in a dose dependent manner to delay the differentiation of CFs induced by AngⅡ.Ligustrazine can inhibit the expression of CaN and downstream signaling molecules NFAT3, which indicates that the inhibition of CFs fibrosis process may be related to the CaN signaling pathway.
Keywords：ligustrazineangiotensinⅡsmooth muscle alpha actinbeta isoform of calcineurinCaN signaling pathwaynuclear fac-tor of activated T lymphocyte 3cardiac fibroblastsSprague Dawley rats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2758-2761 )