Influence of Salidroside on Myocardial Infarctional Size and Apoptosis Index in Rats with Myocardial Ischemia Reperfusion Injury

Liu Xiaodan; Pan Tao

doi:10.3969/j.issn.1672-1349.2016.23.010

Abstract：Objective To observe the effect of salidroside on myocardial size and apoptosis index in rats with myocardial ischemia reperfusion injury (MIRI),to investigate the function of salidroside repel MIRI.Methods Sprague Dawley rats were randomly divided into 6 groups :sham operation group (S group),ischemia reperfusion group (I/R group),salidroside preventive group(first treating with salidroside and then inducing ischemia reperfusion)and salidroside treatment group (first inducing ischemia reperfusion and then treating with salidroside), salidroside preventive + LY294002 group, salidroside treatment + LY294002 group , 6 rats in each group.Both of the salidroside preventive group and salidroside preventive+LY group administered continuously modeling three days before,salidroside treatment group and salidroside treatment +LY group in addition to the salidroside immediate reperfusion.Rats in sham operation group and ischemia reperfusion group(I/R group)received a same volume of 0.9%NaCl.The salidroside preventive+LY group and treatment +LY group with an additional injection of PI3K inhibitor(LY294002)injection slowly 35 min before ligation. Myocardial ischemia sizes were measured by Evans blue and 2,3,5 triphenyltetrazolium chloride (TTC)staining.Myocardium apop-tosis rate was measured by TUNEL.Results Compared with I/R group,in both salidroside preventive and treatment groups,myocar-dial infact size reduced and the apoptosis index lower remarkable (P<0.05),Compared with salidroside treatment group and pre-ventive group ,preventive+LY group and treatment+LY group myocardial infact size increased,apoptosis index increased(P<0.05). Conclusion Salidroside can reduce myocardial infarctional size and apoptosis index in rats with MIRI.