The Role of Voltage dependent Potassium Channel in Geniposide induced Insulin Secretion in Rats
Liu Yunfeng
Ding Yaqin
Zhong Xiangqin
Ren Lele
Bai Tao
Liu Mengmeng
Zhang Yi
Abstract：Objective To explore the role of voltage dependent potassium (Kv)channel in the mechanism of geniposide induced in-sulin secretion in rats. Methods We evaluated the direct effects of geniposide onβ cel function using rat pancreatic islets and dis-persed single cel s. Patchβ clamp recordings were applied to measure Kv channels,action potential duration (APD). The level of in-sulin secretion and the content of cyclic AMP in the culture medium were measured with radioimmunoassay. Results Geniposide (0 to 100μmol/L)potentiated insulin secretion from pancreatic islets in a dose dependent manner under 8.3 mmol/L glucose conditions but not 2.8 mmol/L,and a maximal increase was observed when islets were exposed to geniposide at a concentration of 10μmol/L. The current voltage relationship curve obtained in patch clamp experiments demonstrated that geniposide (0 to 100μmol/L)dose de-pendently decreased the current densities through Kv channels and exerted a maximal inhibitory effect at the concentration of 10μmol/L.What’s more,we detected that geniposide significantly prolonged APD. Conclusion Geniposide can promote insulin secre-tion by inhibiting Kv and prolonging APD in isletβcel s.
Keywords：geniposideinsulin secretionvoltage dependent potassium channelaction potential duration
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2626-2628 )