Effects of Astraglus Polysaccharides on the Cadiocytes Apoptosis Induced by H2 O2 in Neonatal Rat
Shen Yujue
Abstract：Objective To investigate the effects of astraglus polysaccharides (APS)on the cadiocytes apoptosis induced by H2 O2 in neonatal rat. Methods Cadiocytes of neonate rat was cultivated for 72 hours and divided into six groups:normal control group,H2 O2 group,APS (20,40,80μmol/L)plus H2 O2 groups(n=10). Twelve hours after the drugs were given,the survival rate was detected by MTT assay.The cardiomyocytes apoptosis was observed by flow cytometry and the apoptosis rate was calculated.The activity of superoxide dismutate(SOD),catalase (CAT)and the content of malondialdehyde (MDA)in cardiomyocytes were determinted.The content of aspartate aminotransferase(AST),creatine phosphokinase(CPK),lactate dehydrogenase (LDH)in culture medium were detected.The expression of nuclear factor kappa B(NF κB),caspase 3 protein was detected by western blot and Semi quantita-tive analysized.The expression of AKT mRNA,bcl 2 mRNA,Bax mRNA were detected by reverse transcription polymerase chain re-action (RT PCR)and the ratio of bcl 2/Bax was calculated. Results Compared with the H2 O2 group,the survival rate in APS(40, 80μmol/L)plus H2 O2 groups was significantly increased.The cardiomyocytes apoptosis was improved and the apoptosis rate was significantly decreased.The activities of SOD,CAT in cardiomyocytes were significantly increased and the content of MDA was signifi-cantly decreased.The contents of AST,CPK,LDH in culture medium were significantly decreased.The expression of AKT mRNA,bcl 2 mR-NA were significantly up regulated,while the expression of Bax mRNA was significantly down regulated,the ratio of bcl 2/Bax was sig-nificantly increased.The expression of NF κB,caspase 3 protein were significantly down regulated.Al of the difference above were significant (P<0.05 or P<0.01). Conclusion APS had inhibitive effects on neonatal rat cadiocytes apoptosis induced by H2 O2 ,which perhaps related to its effects of improving the activity of antioxidase,depressing oxidative stress,down regulating the expression of NF κB,caspase 3 protein,up regulating the expression of anti apoptotic genes and down regulating the expres-sion of pro apoptotic genes,raising the ratio of bcl 2/Bax.
Keywords：astraglus polysaccharidesH2 O2cardiomyocytessuperoxide dismutatecatalasemyocardial enzymes
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 2616-2621 )