The Influence of Shenshao Capsule Preconditioning on Heart Oxidative Damage in Rats with Myocardial Ischemia Reperfusion Injury
Zhao Guifeng
Huang Xinwei
Wu Liyu
Mao Jingyuan
Abstract：Objective To observe the effect of Shenshao capsule (SSC)preconditioning on myocardial ischemia reperfusion injury (MIRI)in rat heart and the mechanism of oxidative damage and antioxidant capacity.Methods In this study,besides sham group, the left anterior descending coronary artery of other wistar rats were occluded 30 min and reperfused 120 min in MIRI model.Thirty rats were randomly divided into sham group,ischemia/reperfusion group,low and high dose SSC group (250 mg/kg and 500 mg/kg),and atorvastatin 10 mg/kg group,pre treated for 7 days. After the end of the reperfusion,physiological recorder was used to measure the cardiac hemodynamic changes. The contents of serum creatine kinase isoenzyme MB(CK MB)and lactate dehydrogenase (LDH) and the myocardial tissue malondialdehyde (MDA),superoxide dismutase (SOD),glutathione peroxidase (GSH PX)were detec-ted by automatic biochemical analyzer. Results Compared with model group,the contents of serum CK MB and LDH were signifi-cantly lower in low and high dose SSC group and atorvastatin group(P<0.05 or P<0.01). Compared with low dose SSC group,the content of LDH was significantly lower in high dose SSC group and atorvastatin group (P<0.01).Compared with model group,+dp/dtmax value increased significantly in low dose SSC group(P<0.05).LVSP and dp/dtmin value increased (P<0.05),+dp/dtmax value increased signifi-cantly (P<0.01),LVEDP value decreased (P<0.05)in high dose SSC group and atorvastatin group.Compared with model group, the contents of myocardial MDA significantly lower,the contents of SOD and GSH PX increased significantly in low and high dose SSC group and atorvastatin group(P<0.05 or P<0.01).Conclusion SSC pretreatment can inhibit myocardial injury in rats after MIRI,improve cardiac hemodynamic state,inhibit the generation of membrane lipid peroxidation products and improve the body’s ability to resist oxidative damage.
Keywords：Shenshao capsulemyocardial ischemia reperfusion injuryoxidative damagecreatine kinase isoenzyme MB
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2613-2616 )