The Influence of Wenxin Granule on the Cytoskeleton Protein in Ang Ⅱ Induced H9C2 Cel Hypertrophy Model
Ren Xiaomeng
Chen Yu
Li Jie
Wu Lijun
Yang Xinyu
Wu Aiming
Xing Yanwei
Shang Hongcai
Gao Yonghong
Abstract：Objective To study the effect of Wenxin granule (WXG)on the cytoskeleton protein of angiotensin Ⅱ(AngⅡ)induced H9C2 cel hypertrophy model. Methods H9C2 cel s were cultured in vitro,the model of cardiomyocyte hypertrophy was induced by AngⅡ. After dosing WXG with 5 g/L,the morphological changes of the cel s and the cytoskeleton were observe. Results The mor-phological of H9C2 cel induced by AngⅡhad changes,cel transverse tensile,swel ing becomes larger,cel volume vs normal cel s. After with the addition of WXG,H9C2 myocardial cel morphology had been restored,but stil larger than the transverse width of nor-mal cel s. And the discovery of cel morphology were observed under confocal laser scanning microscopy (CLSM),cel morphology was apparent swel ing becomes larger,the length and the width of the cel respectively by 120.32μm± 8.36μm and 51.82μm± 8.62μm increased to 182.67μm± 7.81μm and 92.22μm± 7.56μm. After 24 hours of WXG,H9C2 myocardial cel morphology in AngⅡgroup had been restored,the length and the width of the cel to 160.35μm± 5.66μm,55.87μm± 4.52μm. Conclusion In H9C2 cel model of hypertrophic myocardium induced by AngⅡ,the WXKL can improve the cytoskeletal protein,which plays a role in improving cardiac hypertrophy.
Keywords：cardiac hypertrophyH9C2 cel sangiotensin ⅡWenxin granule
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2609-2612 )