The Influence of Oxymatrine on Oxidative Stress and Apoptosis in Neonatal Rat Impaired by Hypoxia reoxygenation
Chen Shujie
Yang Xiaolong
Wang Xuefen
Chen Chen
Wang Chaozong
Abstract：Objective To investigate the effects of oxymatrine(OMT)on oxidative stress and apoptosis in neonatal rat impaired by hy-poxia- reoxygenation(H/R).Methods Cadiocytes of neonate rats were cultivated for 72 hours and divided into six groups:normal control group,H/R model control group,OMT (20μg/mL,40μg/mL and 80μg/mL)treatment groups and Shuxuening injection(SXN, 100μg/mL)treatment group (n=10). After 6 hours of drugs intervention,the survival rate was detecd.The content of aspartate trans-aminase (AST),creatine phosphokinase (CPK),lactate dehydrogenase (LDH)in culture medium were deteced.The activity of su-peroxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GSH Px)and the content of malondialdehyde(MDA)in car-diomyocytes were determined.The apoptosis was observed by flow cytometry and the apoptosis rate was calculated.The expression of bcl 2 mRNA,Bax mRNA were detected by reverse transcription polymerase chain reaction (RT PCR).The ratio of bcl 2/Bax was calculated.The expression of caspase 3 protein was detected by western blot. Results Compared with the model control group,the survival rate were significantly increased in OMT(40μg/mL,80μg/mL)treatment groups.The content of CPK,AST,LDH in culture medium were significantly decreased.The activity of SOD,CAT in cardiomyocytes were significantly increased and the content of MDA was significantly decreased.The apoptosis rate was significantly decreased.The expression of bcl 2 was significantly up regulated,the expression of Bax and caspase 3 were significantly down regulated.The ratio of bcl 2/Bax were significantly in-creased.The activity of GSH Px was significantly increased(P<0.05).Al of the difference above were significant(P<0.05 or P<0.01).Conclusion OMT had inhibitive effects on oxidative stress and apoptosis of neonatal rat impaired by H/R,which perhaps relat-ed to its effects of improving the activity of antioxidase and regulating the expression of apoptosis related genes.
Keywords：hypoxia reoxygenation injurycardiomyocytesoxymatrineoxidative stressapoptosis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 2366-2370,2371 )