Research of Xiongzhi Dilong Decoction on PKCγ,NMDAR1 in the Trigeminocervical Complex in Migraine Animal
Zhao Yonglie
Hu Kun
Yue Guangxin
Abstract：Objective To study the effects of Xiongzhi Dilong decoction (XDD)on PKCγ,NMDAR1 in the trigeminocervical complex in migraine animal.Methods Healthy male Sprague Dawley (SD)rats were randomly divided into four groups:saline group,migraine model group,sumatriptan group and XDD group,8 rats in each group.The migraine animal model was made by subcutaneous injec-tion of nitroglycerin (NTG).After administered with corresponding drug,specimens was col ected after 4 hours of NTG subcutaneous injection.The protein expression levels of N methyl D aspartate (NMDA)receptor 1(NMDAR1),protein kinase C (PKC)γ,pN-MDAR1(Ser896),pPKCγ(Thr514)in the trigeminocervical complex of migraine animal were assayed by western blot analysis.Re-sults The protein expression level of pPKCγ(Thr514),pNMDAR1(Ser896)in migraine model group were higher than that in saline group(P<0.01).After administration,the protein expression levels of pPKCγ(Thr514),pNMDAR1(Ser896)were significantly de-creased in XDD group and sumatriptan group compared with the migraine model group(P<0.01).Conclusion The conduction of pain signal in migraine is related to the expression of pPKCγand pNMDAR1 in trigeminocervical complex.XDD and sumatriptan can inhibit the protein expression levels of pPKCγand pNMDAR1 in trigeminocervical complex.
Keywords：migraineXiongzhi Dilong decoctionprotein kinase CγN methyl D aspartate receptor 1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2248-2251 )