Experimental Research of Guanmaining Oral Liquid on Atherosclerosis in Apolipoprotein E Gene Knockout Mice
Luo Wen
Zhang Sanlin
Abstract：Objective To explore the effects and its mechanisms of Guanmaining oral liquid (GOL)on atherosclerosis(AS)in apoli-poprotein E gene knockout mice(ApoE / mice).Methods Total y 30 C57BL/6J genetic background ApoE / mice which were 10 weeks old were fed a high fat diet for 12 weeks until the formation of a mature atherosclerotic plaque.They were randomly divided into three groups:model group,GOL group,compound salvia miltiorrhiza group,10 mice in each group. Another 10 wild type normal C57BL/6J mice were used as the blank control group.They were administered for 12 weeks.Blood was extracted from the bulbus ocu-lis.The serum lipid contents were observed with enzyme method.visfatin and interleukin 6 (IL 6)level were detected by enzyme linked immunosorbent assay(ELISA).HE stain was used to observe aortic pathomorphological changes.Results The blank control group showed no distinctive changes of atherosclerosis,the model group had distinctive increase of aortic intima thickness and ather-osclerotic plaque formation.The model group also had high serum levels of total cholesterol(TC),triglyceride(TG)and low density li-poprotcin(LDL),as wel as high visfatin and IL 6 expression .However,high density lipoprotein(HDL)in the model group was lower than the blank control group(al P<0.05).Compared with the model group,the serum levels of TC,TG,LDL C were decreased.Vis-fatin and IL 6 expression were lowered(P<0.05),while HDL C was increased(P<0.05)in Guan maining group and compound salvia miltiorrhiza group,degree of pathological damage was reduced.There was no significant difference in above mentioned indi-cators between GOL group and compound salvia miltiorrhiza group(P>0.05).Conclusion GOL could decrease the serum levels and the expression of visfatin and IL 6 of ApoE / mice.It can inhibit the development of atherosclerosis by regulating serum lipids, suppressing inflammation factors,which is the possible mechanism of stabilizing the atherosclerotic plaque.
Keywords：atherosclerosisapolipoprotein E gene knockout micevisfatininterleukin 6Guanmaining oral liquid
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2240-2243 )