Establishment and Evaluation of Rats Model of Diabetic Myocardial Ischemia reperfusion Injury Induced by Streptozotocin
Wang Xiang
Wang Xiaopeng
Han Chongfang
Luo Min
Yu Jing
Zhang Jianwen
Yang Wenqu
He Jiandong
Abstract：Objective To establish the diabetic myocardial ischemia reperfusion injury model in rats,to provide a platform for inter-vention experiment.Methods Forty five healthy male Sprague Dawley (SD)rats were randomly divided into three group normal control group (Sham group),model of diabetes (DM group),diabetes and myocardial ischemia reperfusion injury model group (DM+I/R group).Myocardial ischemia was induced by 30 min occlusion of left anterior descending branch of coronary artery fol owed by 120 min reperfusion .The rats of DM group and DM+I/R group by intraperitoneal were injected Streptozotocin (STZ)(60 mg/kg)to es-tablish diabetic rats model.After 1 week,2 weeks,3 weeks and 4 weeks,the injection of STZ to observe the rats fasting plasma glu-cose,body weight,urine volume,water quantity and the general situation.The myocardial infarction area was measured by tripheny-ltetrazolium chloride TTC.HE staining and myocardial pathology were observed under light damage.Results Compared with the sham group,the fasting blood glucose value in DM group and DM+I/R group were significantly increased after intraperitoneal injec-tion of STZ,the weight was significantly reducted,and the urine volume and water quantity were increased significantly .Compared with DM group,the myocardial infarction area of DM+I/R group was obviously increased,myocardial pathology injury aggravated. Conclusion:The experiment method of diabetic myocardial ischemia reperfusion injury in rats model of success rate is higher,has good stability,and applied research.
Keywords：myocardial ischemia reperfusion injurydiabetesstreptozotocin
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1996-1998 )