Regulation of Yangxinshi Tablets in the Macrophage Polarization and Activation
Liang Hou
Lu Yingjin
Ding Yanchun
Abstract：Objective To explore the anti atherosclerotic mechanism of Yangxinshi tablets(YXST),we stimulated THP 1 derived macrophages with YXST and observed its effects on macrophage polarity switch and activation.Methods Human monocytic cel line THP 1 were differentiated into macrophages by stimulated with 160 nmol/L phorbol 12 myristate 13 acetate (PMA)for 24 h.Then THP 1 derived macrophages were stimulated by different concentrations of oxidized low density lipoprotein (ox LDL)for 24 h, which were 10mg/L,25mg/L and 50mg/L,and blank was used as control.The secretion of monocyte chemotactic protein 1 (MCP 1) and macrophage migration inhibition factor (MIF)were detected by ELISA,and the membrane molecule CD16 and CD68 were tested by flow cytometry.THP 1 derived macrophages were pretreated with different concentrations of YXST (10 mg/L,50 mg/L and 100 mg/L)12 h.Then we stimulated these cel s by ox LDL (50 mg/L)for 24 h and detected the secretion of MIF,MCP 1 and the ex-pression of CD16 and CD68 again.Results The ox LDL up regulatet the secretion of MCP 1 and MIF as a dose dependent manner in THP 1 derived macrophages.With the increasing concentration of ox LDL,the secretion of MCP 1 and MIF increased, 50mg/L group reached their secretion peaks after 24h(MCP 1:29.30± 1.48 pg/mL vs.control 5.71± 1.94 pg/mL;MIF:18.67± 0.15 ng/mL vs.control 4.61± 0.40 ng/mL;P<0.01).The expression of CD16 and CD68 were varied with different ox LDL concentrations.The mean fluorescence intensity of CD16 and CD68 was significantly reduced in 50mg/L group (CD16:26.9 vs.control 29.8;CD68:22.3 vs. control 25.2).YXST inhibited the expression of MCP 1 and MIF induced by ox LDL as a dose dependent manner.With the increas-ing concentration of Yangxinshi,the secretion of MCP 1 and MIF decreased.The 100mg/L group declined mostly(MCP 1:9.95± 2.09 pg/mL vs.control 33.30± 2.37 pg/mL;MIF:4.85± 0.12 ng/mL vs.control 18.65± 0.15 ng/mL;P<0.01).Yangxinshi change the ex-pression of CD16 and CD68 reduced by ox LDL as a dose dependent manner,the mean fluorescence intensity of CD16 and CD68 was significantly up regulated in 100 mg/L group (CD16:96 vs.control 26.9;CD68:91.6 vs.control 22.3).Conclusion YXST may ex-ecute its anti atherosclerotic effect by inhibiting macrophage inflammatory activation and affecting macrophage polarity switch.
Keywords：atherosclerosismacrophage polarizationYangxinshi tabletsmonocyte chemotactic protein 1macrophage migration in-hibition factor
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1722-1726 )