Establishment of Sprague Dawley Rat Model with Chronic Heart Failure and Yang Deficiency Syndrome and Index Determination
Yang Zhe
Chai Hongjia
Wang Dawei
Abstract：Objective To establish the animal model with chronic heart failure (CHF)and yang deficiency syndrome.Methods The experimental animals were randomly divided into yang deficiency model group and sham operation group.The yang deficiency models were established by abdominal aortic coarctation method.The Sprague Dawley (SD)rat models with CHF were established by ab-dominal aorta coarctation.After normal feeding for 1 week,rats placed in 2 to 4 degrees Celsius freezer in 2 hours a day at the same time,lasting 4 weeks.Sham operation SD rats were established by abdominal incision after surgical thread wear abdominal aorta.After 4 weeks,the behavior of rats,body temperature and platelet aggregation rate were observed.The rat myocardial tissue was observed by electron microscope examination.Results The study showed that rats appeared in succession to a drop in body temperature,fur and erect,huddled together,dispirited,slow,dorsal edema and tail cool dim etc.At the same time,after the establishment of the mod-el of yang deficiency model rats body temperature was significantly lower than that of the sham operation group,platelet aggregation rate increased significantly (P<0.05),mean platelet volume (MPV)and platelet distribution width (PDW)increased significantly (P<0.01), the myocardial mitochondria cavitation,cristae dissolved and disappeared,muscle fiber loose and above were confirmed type of de-ficiency of both heart failure model was made successful y.Conclusion The model simulates symptoms characteristic of CHF with yang deficiency syndrome in a certain extent.The results of biological features and objective indexes after repeated experiments are consistent.
Keywords：chronic heart failureyang deficiency syndromemean platelet volumeplatelet distribution widthplatelet aggregation rate
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1593-1596 )