Effect of Qigui Yimailing Decoction on Cox 2 in Rat Model with Acute Myocardial Ischemia reperfusion Injury

Xu Jingyu; Zhao Long; Zhang Chaoyue

doi:10.3969/j.issn.1672-1349.2016.10.008

Abstract：Objective To explore the effect Qigui Yimailing decoction(QYD)on Cox 2 in rat myocardial tissue with acute myocardial ischemia reperfusion.Methods Fifty SD male rats were randomly divided into 5 groups and blank control group(n=10)after 7 days physiological saline irrigation,don’t do any processingthe sham operation group(n=10)after 7 days physiological saline irrigation-take the thoracotomy to exposeleft coronary artery of the rats,threading but do not make ligation,the rest of 30 rats were divided into pure ischemia reperfusion group,QYD low dose group and QYD high dose group,and acute myocardial infarction (AMI)models were caused by ligation of the left anterior descending branch of the coronary artery,after the success of the building,to the physiological saline,QYD (high and smal dose,1.2 g/kg and 0.6 g/kg)respectively,whose irrigation served 7 days in a row during the morning.Ab-dominal aorta in pursuance of 5 mL,after centrifugation,get the upper serum,with double antibody sandwich method enzyme linked immunoassay to determine the expression of cox 2.Results The cox 2 of the pure ischemia reperfusion group were significantly higher than the blank control group’s and the sham operation group’s(P<0.05).Compared with pure ischemia reperfusion group, cox 2 overflow of QYD group significantly reduced in rats serum,and the effect of high dose group was more significant.Conclusion Cox 2 has a high level expression in acute myocardial ischemia reperfusion,which prompted inflammation is involved in acute myocardial ischemia reperfusion,QYD can decrease the cox 2 protein expression level and reduce the pure ischemia reperfusion group (I/R)myocardial inflammation,to protect myocardium.