The Influence of Xiongzhi Dilong Decoction on the Expressions of c fos and CGRP Immunoreactive Neurons in Brain Tissues of Mi-graine Model Rats
Zhao Yonglie
Wang Yulai
Yue Guangxin
Abstract：Objective To observe the infcuence of Xiongzhi Dilong decoction(XDD)on the expressions of c fos and calcitonin gene related peptide(CGRP)immunoreactive neurons in brain tissues of migraine model rats,and to explore its mechanism.Methods For-ty eight healthy male Sprague Dawley(SD)rats were divided randomly and equal y into 4 groups:saline group,migraine model group,sumatriptan group,XDD group.The rats were pretreated with sumatriptan,XDD or saline before received a subcutaneous injec-tion of 10 mg/kg nitroglycerin or saline.After the rats received a subcutaneous injection 4 h and 6 h,the brain tissue samples were col-lected.Immunohistochemistry was applied to detect c fos and CGRP immunoreactive neurons in trigeminal nucleus caudalis(TNC) and periaqueductal gray(PAG).Results Immunoreactive positive neurons in trigeminal nucleus caudalis:c fos immunoreactive neurons and CGRP immunoreactive neurons in XDD group were decreased at both 4 h and 6 h,and there was statistical difference comparing with the migraine model group(P<0.01).Immunoreactive positive neurons in periaqueductal gray(PAG):c fos immuno-reactive neurons and CGRP immunoreactive neurons in XDD group were decreased at both 4 h and 6 h,and there was statistical difference comparing with the migraine model group(P<0.01)as wel .Conclusion XDD can reduce the NTG induced c fos im-munoreactive neurons in trigeminal nucleus caudalis,and attenuate the activatin of trigeminovascular system,can reduce the CGRP immunoreactive neurons in periaqueductal gray as wel ,and inhibit nociceptive transmission.XDD can reduce the CGRP immunoreac-tive neurons in trigeminal nucleus caudalis and periaqueductal gray.
Keywords：migraineanimal modelsXiongzhi Dilong decoctionspinal trigeminal nucleusperiaqueductal gray mattercalcitonin gene related peptide
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 957-959,960 )