Mechanisms of Angiotensin(1 7)on Aortic Endothelial Cel Injury Induced by Oxidized Low Density Lipoprotein in Rats
Du Ninghui
Yang Zhiming
Yang Huiyu
Abstract：Objective To investigate the effects of angiotensin (1 7)[Ang (1 7)]on expression levels of lectin like oxidized low density lipoprotein receptor 1 (LOX 1),intercel ular adhesion molecule 1(ICAM 1)and vascular cel adhesion molecule 1 (VCAM 1)in SV40 transformed aortic rat endothelial cel s (SVAREC)induced by oxidized low density lipoprotein(ox LDL),and to explore its possible mechanism.Methods Cultured endothelial cel s of rats were randomly divided into ox LDL group and the ox LDL+ Ang (1 7)group.The cel proliferation was detected by experimental Cel Counting Kit (CCK 8).The expression levels of LOX 1,ICAM 1,VCAM 1 mRNA were detected by fluorescent quantitative reverse transcription polymerase chain reaction (RT PCR)method.Results Ox LDL could inhibit SVAREC cel proliferation in a dose dependent manner,and there was statistical y significant compared with the control group (P<0.05).Compared with control group,SVAREC cel growth inhibition rate was reduced in Ox LDL+ Ang(1 7)group.With the increase of ox LDL concentration,LOX 1,ICAM 1 and VCAM 1 mRNA expression in-creased,and in a dose dependent manner,and there was statistical y significant compared with the control group(P<0.05).With the increase of Ang(1 7),LOX 1,ICAM 1 and VCAM 1 mRNA expression decreased in Ox LDL+ Ang(1 7)group.Conclu-sion SVAREC ox LDL can damage cel s and LOX 1,ICAM 1 and increased VCAM 1mRNA expression and Ang(1 7)inhibi-tion of ox LDL of the reaction,and through the LOX 1,ICAM 1 and VCAM 1mRNA play a role.
Keywords：angiotensin (1 7)oxidized low density lipoproteinlectin like oxidized low density lipoprotein receptor 1SV40 transformed aortic rat endothelial cel svascular cel adhesion molecule 1intercel ular adhesion molecule 1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 833-837 )