Regulative Effects of Yiqifumai Injection on the Activity of Matrix Metal oproteinase in Rats with Chronic Heart Failure
Zhang Qiuyue
Wang Baohe
Liu Weishuang
Deng Yafang
Abstract：Objective To investigate the regulative effect of Yiqi Fumai injection(YFI)on the activity of matrix metal oproteinase (MMPs)and tissue inhibitor of metal oproteinase(TIMPs)in rats with chronic heart failure(CHF),and explore the regulation of YFI on MMPs and its correlation with the reconstruction of myocardial extracel ular matrix(EMC).Methods One hundred Wistar rats were randomly divided into sham group (n=10)and model group(n=90).The CHF rat model was established by abdominal aortic ban-ding.After 8 weeks of operation,rats were divided into 6 groups:blank model group,captopril group,Shengmai injection(SMI) group,YFI groups(high ,medium ,low dosage).After 14 days,the myocardial pathological changes were observed by hematoxy-lin eosin (HE)staining.The contents of MMP 2,MMP 3,MMP 9,TIMP 1 and TIMP 2 in myocardial tissue were detected by western blotting.Results Compared with sham group,myocardial interstitial col agen fibers proliferated,EMC structure changed,fo-cal fibrous scars were visible in blank model group.The contents of MMP 2,MMP 3,MMP 9 and MMP 2/TIMP 2 ratio increased while the contents of TIMP 1 and TIMP 2 decreased in blank model group.Compared with blank model group group,the light and moderate proliferation of col agen fibers appeared around myocardial muscle bundle and vessels in captopril group,SMI group,YFI groups.The contents of MMP 2,MMP 3,MMP 9 and MMP 2/TIMP 2 ratio decreased while the contents of TIMP 1 and TIMP 2 increased in captopril group,SMI group,YFI groups.The contents of MMP 2,MMP 3,MMP 9 in YFI groups were lower than that in SMI group(P<0.05).The content of MMP 2 in YFI low dosage group was higher than that in captopril group(P<0.05). The contents of TIMP 2 in YFI groups were higher than that in SMI group(P<0.05).The content of TIMP 1 and MMP 2/TIMP 2 ratio in YFI groups were lower than that in captopril group(P<0.05).Conclusion The myocardial interstitial col agen proliferation and col agen structure changes induce the reconstruction of EMC in rats with CHF,which is relevant to the enhancement of MMPs activity.YFI can inhibit MMPs activity and improve myocardial EMC remodeling by by lowering the content of MMP 2,MMP 3,MMP 9, increasing the content of TIMP 1,TIMP 2,maintaining MMP 2/TIMP 2 homeostasis in rats with CHF.
Keywords：chronic heart failurematrix metal oproteinasetissue inhibitor of metal oproteinaseYiqi Fumai injectionwestern blotting
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 825-829 )