Compatibility Effects of Danshen and Gualou on Hepcidin and Its Related Signal Pathway in Mice with Hyperlipidemia
Wang Lin
Ma Xiaojuan
Sun Mingyue
Miao Yang
Yin Huijun
Abstract：Objective To investigate the compatibility effects of Danshen and Gualou on hepcidin and its related bone morphogenetic protein(BMP)/Smad signal pathway in mice with hyperlipidemia induced by high fat diet,and to explore its possible mechanisms. Methods Fifty female Fpn1 Tek cre mice were randomly divided into control group and model group.Eigh mice were fed with stand-ard diet as control group,others were given high fat diet for 8 weeks to establish the hyperlipidemia models.After 8 weeks,the es-tablished mice were randomly divided into five groups and were intragastrical y administered normal saline,atorvastatin suspension, Danshen decoction,Gualou decoction,Danshen Gualou decoction for 2 weeks,respectively.Blood was col ected in sterile tubes,liver were removed and sacrificed by cervical dislocation,Serum lipids parameters were measured with an automated analyzer.The levels of interleukin 6 (IL 6),hepcidin,malonaldehyde(MDA),superoxide dismutase (SOD)were determined by enzyme linked immu-nosorbent assay.The liver expression position of bone morphogenetic protein 6 (BMP 6)was analysed by immunohistochemical method.Western blotting was to test the expressions of phosphorylation of Smad 1/5/8 and stat 3.Results Compared with the control group,the levels of total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL C),hepcidin were in-creased in model groups(P<0.05).Compared with model group,the levels of hepcidin and BMP 6 were decreased in treatment groups(P<0.01 or P<0.05).Compared with atorvastatin group,the expressions of phosphorylation of stat 3 and Smad were de-creased in Danshen Gualou decoction group(P<0.05).Compared with Danshen decoction group,the levels TC and hepcidin were decreased in Danshen Gualou decoction group(P<0.05).Compared with Gualou decoction group,the levels of TG,TC and hepcidin were decreased in Danshen Gualou decoction group(P<0.05).Conclusion Danshen and Gualou compatibility can regulate the ex-pression of hepcidin and its related BMP/Smad signal pathway in mice with hyperlipidemia.
Keywords：hyperlipidemiahepcidinDanshenGualoubone morphogenetic protein/Smad signal pathwaymice
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 699-704 )