The Possible Mechanism of Recombinant Human Erythropoietin Improving the Cognitive Function in Rats with Vascular Dementia
Yang Qingjie
Guo Junhong
Abstract：Objective To investigate the possible mechanism of recombinant human erythropoietin (rhEPO)to improve the cognitive function in rats with vascular dementia.Methods By Morris water maze qualified after screening,thiry clean male Sprague Dawley (SD)rats were randomly divided into five groups:Sham group,group 2(2VO group),group 3(2VO+rhEPO group),group 42VO+rhEPO+AG490(Janus kinase 2 inhibitor)group and group 5(2VO+AG490 group).Six rats were in each group.The bilateral occlu-sion of common carotid arteries in SD rats was adopted to established the vascular dementia model.Rats respectively were disposed by intraperitoneal injection of AG490(5 mg/kg)or the same quantity of DMSO AG490 solvent after chronic ischemia for 3 days.After 30 minutes,rats respectively undergone intranasal delivery rhEPO at the dose of 150 U/125μL or equivalent volume of saline at the sametime and administered once a week for a total of eight times.After the 8 weeks ,the Morris water maze was explored to ob-served the spatial learning and memory capability.Morphology changes of hippocampal CA1 nerve cel s were observed by Hematox-ylin Eosin (HE)staining.Results Behavioral tests results showed that the mean escape latency,with the increase of training days,was significantly shortened in rats.The average escape latency period in group 3 was lower than that in group 2,group 4 and group 5(P<0.05).The number of cross platform in group 3 was obviously higher than that in group 2,group 4,group 5(P<0.05).HE staining showed that cel s were ordered in the hippocampal CA1 area and with normal cel s form in Sham group.The cel s of hipp-ocampal CA1 area were disordered and the cel body contraction,nuclear hyperchromatic,nucleus pycnosis and nuclear dissolved could be visible in group2.The cel s of hippocampal CA1 area arranged neatly and the cel structure closed to normal cel s,a few of contractible cel s could be seen occasional y in group 3.The morphological changes of hippocampal CA1 area in group 4 and group 5 were similar to group2.Conclusion The rhEPO could improve the cognitive function in rats with vascular dementia,its mechanism may be related to activation of JAK2 STAT3 pathway.
Keywords：AG490vascular dementiaerythropoietinJAK2 STAT3
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1940-1943 )