The Influence of Yiqi Huoxue Drugs on the Expression of Connexin 43 and Ventricular Fibril ation in Rats with Myocardial Infarction

Wu Aiming; Lou Lixia; Zhang Dongmei; Chai Limin; Nie Bo; Zhao Mingjing

doi:10.3969/j.issn.1672-1349.2015.16.008

Abstract：Objective To study the effects of Yiqi Huoxue drugs(YQHX,combine Shengmai injection with Xuesaitong injection)on the expression of connexin 43(Cx43)and ventricular fibril ation in rats with myocardial infarction.Methods Model rats of myocardial in-farction were established by occlusion of the left anterior descending coronary artery,the sham operation group was without occlu-sion.After operation rats were randomly divided into sham operation group,model group,YQHX group and captopril group. YQHX [Shengmai injection 3.51 mL/(kg· d)and Xuesaitong injection 17.54 mg/(kg· d)]were administered intraperitoneal y.Captopril[4.39 mg/(kg· d)]and distil ed water (for the sham operation and model groups)were administered oral y.The course of treatment was 4 weeks.The expressions of Cx43 mRNA of myocardium in left ventricular infarct border zone were detected by real time fluorescent quantitative polymerase chain reaction after treatment.The expression of Cx43 protein was observed by western blot.The incidence of ventricular fibril ation was recorded by electric stimulation in vivo.Results Compared with sham operation group,the expressions of Cx43 mRNA and protein decreased remarkably in the model group (P<0.05 or P<0.01).Compared with model group,the expres-sions of Cx43 mRNA and protein increased significantly in YQHX group and captopril group (P<0.05 or P<0.01).The incidence of ventricular fibril ation was the highest in model group.The incidence of ventricular fibril ation reduced significantly in captopril group and YQHX group(P<0.05).Conclusion Shengmai injection and Xuesaitong injection can reduce the occurrence of ventricular fibril-lation after myocardial infarction.It is related to improving the expressions of Cx43.