Regulatory Effects of Astragaloside on Myocardial Injury and Cel Autophagy Induced by Ischemia reperfusion in Rats
Huang Li
Wang Dawei
Yan Xia
Huang Weixin
Abstract：Objective To explore the protection effects of astragaloside on injured myocardium induced by ischemia reperfusion and the relationship between myocardium cel autophagy and astragaloside.Methods The model of myocardial ischemia reperfusion was established by legating the left anterior descending coronary of rats.And,The changes of cardiomyocyte autophagy and injury in ischemia model after being treated with medicated serum were observed by molecular biology and immunocytochemical methods.Re-sults The ischemia reperfusion injury induced the severe myocardium local necrosis,wave shape change and blockbuster inflam-mation cel s infiltration.Compared with sham operation group,lesion area increased clearly(P<0.05).The area of myocardium local necrosis and inflammatory cel infiltration reduced obviously in low or high dose group of astragaloside(P<0.05).The result of west-ern blot test showed the expression of beclin 1 in cardiomyocytes was activated by ischemia reperfusion injury(P<0.05).The ex-pression of beclin 1 in cardiomyocytes induced by ischemia reperfusion injury was decreased in the low or high dose group of astra-galoside(P<0.05).Conclusion To some degree,astragaloside can reduce the area of injured myocardium induced by ischemia reperfusion.The mechanism may be by regulating the signal transduction pathway of beclin 1 cel related with autophagy.
Keywords：myocardiumautophagyischemia reperfusionBeclin 1
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 752-754 )