Effects of Sangqi Qingxuan Granule on Cardiomyocyte Apoptosis in Spontaneously Hypertensive Rats
Li Lin
Feng Run
Huang Li
Abstract：Objective To observe the inhibitory effects of Sangqi Qingxuan granule(SQG)on hypertensive myocardial remodeling,and investigate its possible mechanism.Methods Eight only 4 weeks of male WKY rats and 16 only 4 weeks male spontaneously hyperten-sive rats(SHR)were selected.WKY rats as the control group,SHR were randomly divided into model group and SQG treatment group,each group of eight.Rats were executed after the treatment of 16 weeks.The tail artery systolic pressure,diastolic blood pres-sure,average pressure were measured by tail set method.The cardiac weight index was observed.The myocardial cel s apoptosis was detected by DNA nucleotides terminal transferase mediated Nick end labeling method (TUNEL)staining method.The B lymphocyte tumor 2 (Bcl 2),Bcl 2 associated protein X(Bax),activated caspase 3 (cleaved caspase 3)in myocardial tissue were detected by protein immunoblot method.Results Compared with WKY group,the levels of systolic blood pressure,heart weight index,myocar-dial cel apoptosis,Bax and cleaved caspase 3 were decreased while the level of Bcl 2 was increased in SQG treatment group. Conclusion SQG can inhibit the blood pressure,reduce the myocardial hypertrophy in SHR.Its mechanism may be related to inhibi-ting myocardial apoptosis in SHR.
Keywords：spontaneously hypertensive ratsSangqi Qingxuan granulecardiomyocyte apoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 743-745 )