Correlation Between Thrombin and Inflammatory Factor in Rats with Focal Cerebral Ischemia
Wen Guo
Deng Yihui
Liu Wenhua
Tan Hongyu
Li Dingxiang
Abstract：Objective To observe thrombin and its receptor (PAR 1)and inflammation factor monocyte chemotactic protein (MCP 1), and nuclear factor kappa B(NF kappa B)’s time variation process and their correlation in rats after focal cerebral ischemia.Methods Line plug method to establish the left middle cerebral artery occlusion (MCAO)rat model,respectively in each time point (ischemia of rats after 1 h,6 h,24 h,48 h,72 h),the behavior score,then beheaded in brain,real time fluorescent quantitative PCR (real time PCR)method to detect brain thrombin,and MCP 1 PAR 1,the NF kappa B mRNA expression.Results After ischemia in rats, Thrombin mRNA,PAR 1 mRNA,NF kappa B mRNA and MCP 1 mRNA began increased significantly after making touch,and reach to peak in 24 h,then gradual y decline.Conclusion After cerebral ischemia,the expression of inflammation factors increased within the brain tissue,So did the expression of thrombin.So thrombin may promote inflammation and damage brain cel s by induce brain tissue express and produce inflammation factors.
Keywords：focal cerebral ischemiathrombinthrombin receptornuclear factor kappa Bmonocyte chemotactic protein
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 481-483 )