Protective Effect of Total Isoflavones from Pueraria Lobata on Vascular Endothelial Function in Ovariectomized Rat
Zhang Baorong
Tan Yingying
Zhang Qi
Abstract：Objective To explore the mechanism and effect of total isoflavones from pueraria lobata (TIP)on vascular endothelial function in ovariectomized rat.Methods The female Sprague Dawley (SD)rats underwent bilateral oophorectomy were fed 150 mg/kg and 50 mg/kg of TIP for 12 weeks.Serum estradiol (E2)and endothelin (ET)content were measured by enzyme linked im-munosorbent assay,and serum nitric oxide (NO)levels were detected by nitrate reductase assay.Acetylcholine induced endotheli-um dependent vasodilation and sodium nitroprusside induced reaction were observed in isolated aortic rings in each group rats. Endothelial nitric oxide synthase (eNOS)expression was observed by western blot in the endothelial tissue samples of each group rats.Results Compared with the sham group,the serum E2,ET and NO levels in ovariectomized rats had a significant changes, and its arterial rings significantly alter the diastolic function of acetylcholine,but had no effect on the relaxation of sodium nitroprus-side.After giving the low and high dose of TIP in ovariectomized rat,there were significant differences in serum E2,ET and NO lev-els and endothelial function when compared with ovariectomized rats (P<0.05).In addition,TIP increased the expression of eNOS in endothelial tissue of ovariectomized rat.Conclusion TIP has a role in protecting vascular endothelial function in ovariectomized rat,its mechanism associated with estrogen like effects,increasing eNOS expression and reduce the level of ET.
Keywords：total isoflavones from pueraria lobataendothelial functionestrogenovariectomized rat
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 309-311 )