Effect of Shexiang Baoxin Pil on Vascular Calcification in Model Rats

Zhang Xusheng; Zhu Pingxian; Huang Zhanjun; Cai Bozhi; Zhang Xin; Zhang Yonggang

doi:10.3969/j.issn.16721349.2015.01.019

Abstract：Objective To investigate the effect and possible mechanism of Shexiang Baoxin Pil (SBP)in aorta of vascular calcification model rats.Methods Seven week old male Sprague Dawley (SD)rats were randomly divided into normal group,calcification group and calcification plus SBP group (al n= 6).On the first day in calcification and calcification plus SBP group,vascular calcifi-cation was induced by a single dose intramuscular injection of vitamin D3 (300 000 U/kg)in the morning plus two doses of nicotine (25 mg/kg dissolved in peanut oil )gavage in the morning and in evening.The next day after calcification induction,rats in SBP group were gavaged with SBP[14 mg/(kg· d)]for four weeks.Vascular calcification was confirmed by Von Kossa staining.Calcium content and alkaline phosphatases (ALP)activity were detected by calcium assay kit and ALP detection kit respectively.The contents of plasma monocyte chemotactic protein 1 (MCP 1)were determined by radioimmunoassay,and the expressions of correspond-ing receptors were determined by immunohistochemistry.Results Von Kossa staining showed that there were mass black granules deposited in aortic wal of the vascular calcified rats.Calcium content and ALP activity in calcified group were increased significantly than those in the control group (P<0.01).Meanwhile,MCP 1 contents in plasma and corresponding receptor level in calcified group were up regulated significantly compared with those in the control group(P<0.01).In addition,SBP diet could reduce the degree of vascular calcium contents,ALP activity and MCP 1 content.Conclusion SBP significantly protects the aorta against cal-cification partly through reducing MCP 1 content and ALP activity.