The Changes of COX 2 Gene Expression after Hypoxic ischemic Brain Damage in Neonatal Rats Receiving Mild Hypothermia Intervention and Its Significance
Abstract：Objective To observe the changes of cycloxygenase 2(COX 2)gene expression in brain tissue after hypoxia is-chemia brain damage (HIBD)and receiving mild hypothermia intervention.Methods One hundred and twenty seven day old neo-natal Sprague Dawley(SD)rats that adopted Rice method to establish the model of HIBD and were randomly divided into three groups:Sham operated group (n= 40),normal temperature group (n= 40)and mild hypothermia (n= 40)group.The experimen-tal groups were further divided into five sub groups (n= 8 in each group)based on different time points after HIBD (1 h,6 h,12 h, 24 h,48 h)respectively.Fluorescence quantitative PCR (PT PCR)technique was used to determine the expression changes of COX 2 mRNA in brain tissues.Results COX 2 mRNA expressed had no significant change in sham operated group.The expres-sion of normal temperature group increased at 6 h after HIBD,24 h to the peak level,24 h to 48 h gradual y increased(P<0.05).The COX 2 mRNA expression in mild hypothermia group was significantly lower than that in HIBD group at each time points (P<0.05). Conclusion COX 2 participated in HIBD of neonatal rats.Mild hypothermia intervention could reduce the expression of COX 2 mRNA in brain tissue after HIBD.Therefore,mild hypothermia intervention at the appropriate time window had some cerebral protec-tion effects on the brain tissues of neonatal rats after HIBD.
Keywords：hypoxia ischemia brain damagemild hypothermiacycloxygenase 2
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1540-1542 )