The Difference and Significance of Intracel ular Lipid Contents in M1 and M2 Macrophages and Concentrations of TNF αand IL 10 Secreted by M1 and M2 Macrophages after Stimulating with ox LDL

doi:10.3969/j.issn.16721349.2014.12.049

Abstract：Objective To investigate the differences and significance of intracel ular lipid contents in M1 and M2 macrophages and concentrations of TNF αand IL 10 secreted by M1 and M2 macrophages after stimulating with oxidized low density lipopro-tein(ox LDL).Methods Monocytes were isolated from bone barrow cel s of C57BL/6 murine and were induced into macrophages by M CSF.Macrophages randomly al ocated into two groups and induced with interferon gamma(IFN γ)and Interleukin 4(IL 4)to establish M1 and M2 macrophages,respectively.RT PCR was used to quantitative analysis Arg 1 expression which is M1 macro-phages marker and iNOS expression which is M2 macrophages marker.Then M1 and M2 macrophages were incubated with 40mg/L ox LDL for 48 h,accumulation of lipid was quantid by Oil Red O staining and the concentrations of cytokine IL 10 and TNF αin cel culture supernatants were measured by ELISA.Results Expression levels of Arg1 measured by RT PCR were lower in IFN γstimulated macrophages(M1 macrophages)than IL 4 stimulated macrophages(M2 macrophages).Conversely,expression levels of iNOS was higher in IFN γstimulated macrophages. Lipid content in M1 and M2 macrophages was higher than those in control group,lipid content in M1 macrophages was higher than M2 macrophages.ELISA results indicate that supernatant concentrations of TNF αand IL 10 were higher than those in control group,the supernatant concentrations of IL 10 in M1 macrophages was lower than M2 macrophages(P<0.05).In contrast,the supernatant concentration of TNF αin M1 macrophages was higher than M2 mac-rophages(P<0.05).Conclusion M1 macrophages possibly increase the ability of cholesterol intake by secretion of TNF αand play the role in proatherogenic functions,while M2 macrophages may reduce cholesterol phagocytosis by secretion of IL 10 and play the role of antiatherogenic functions.